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KMID : 0370219960400010041
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Yakhak Hoeji
1996 Volume.40 No. 1 p.41 ~ p.45
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Analysis of Saikosaponins by HPLC with Photoreduction Fluorescence Detection
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신영근/Shin YG
조경희/권수진/도영미/황귀서/박정일/박만기/Cho KH/Kwon SJ/Do YM/Hwang GS/Park JH/Park MK
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Abstract
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A high performance liquid chromatography using photoreduction fluorescence detection was described for the analysis of saikosaponins. Saikosaponins were separated on an NH2 column using acetonitrile and aqueous 2-tert-butylanthraquinone(t-BAQ) as mobile phase. Column effluent was passed through a 40cm PTFE capillary tube coiled around a 10W UV lamp to reduce t-BAQ to a highly fluorescent dihydroxyanthracene derivative which was detected by a fluorescence detector. The optimal concentration of t-BAQ was found to be 6X10-5M and the optimal reaction time to be 2 seconds. The detection limit for saikosaponin a and d by this method was found to be about 280ng and 80ng. The dynamic linear range was over two orders and the correlation coefficient of the calibration curve of them was 0.998.
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